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Medical Gas Systems: Tanks and Pipelines

Key Points

  • Hospitals deliver the medical gases oxygen (O2), air, nitrous oxide (N2O), and carbon dioxide (CO2) to operating rooms at approximately 50 pounds per square inch (psi) through a central pipeline system that uses gas-specific outlets and the diameter index safety system (DISS) to prevent misconnections.
  • O2 is stored as a gas in E-cylinders at room temperature. A full O2 E-cylinder contains 660 L at 2000 psi, and the volume of O2 in the cylinder is directly proportional to the pressure in the cylinder.
  • N2O is stored predominantly as a liquid in E-cylinders at room temperature. A full cylinder has a volume of 1,590 L and a pressure of 745 psi. The volume will not correlate directly with the pressure until N2O exists solely in its gaseous form, which occurs when approximately 250 L (16%) remain in the tank.

Pipeline Supply, Safety Features, and Complications

  • Hospitals use a pipeline system to deliver O2, air, N2O, and CO2 from a central gas supply to the operating room at approximately 50 psi.1
  • Medical gas pipelines are typically made of seamless copper tubing.2
  • Gas-specific outlet connections are located in the wall or ceiling of the operating room.
    • The DISS provides noninterchangeable threaded connections for each specific gas line to prevent the wrong gas from being connected to a delivery site.

Figure 1. Diameter index safety system (DISS). Used with permission from Binda DD et al. Hospital oxygen supply: Videos in Clinical Anesthesia. Anesth Analg. 2024.3

  • Complications of pipeline systems include excessive pipeline pressures, inadequate O2 pressure, and pipeline crossovers.1
  • Inadequate O2 pressure can occur when hospital construction projects cause pipeline damage.
    • A low O2 pressure alarm will sound once the O2 pressure falls below 30 psi.2,4
    • If low O2 pressure occurs, the E-cylinder should be opened, and the O2 pipeline source should be assessed to determine the cause of the pressure loss.5
    • If the E-cylinder was already open when the O2 pipeline failed, the low O2 pressure alarm would not sound until all the gas in the E-cylinder had been used.5 Therefore, it is important to keep the E-cylinder closed during standard pipeline use.5
  • A pipeline crossover can occur through the delivery of gas from an incorrect central gas tank or the connection of a gas pipeline to an incorrect gas inlet and can cause patient death if not promptly recognized.
    • If a pipeline crossover is suspected, the backup cylinder must be opened and the pipeline supply disconnected.1

Figure 2. Nitrous oxide, oxygen, and air valves for an operating room. Used with permission from Binda DD et al. Hospital oxygen supply: Videos in Clinical Anesthesia. Anesth Analg. 2024.3

  • Please see the A&A Videos in Clinical Anesthesia on Hospital Oxygen Supply for more details. Link

Medical Gas Storage: E-cylinder Tanks

  • O2, air, and N2O are often stored in E-cylinder tanks.
    • These E-cylinders can be used if the pipeline fails.
    • While there is no universal standard for cylinder colors, in the United States, O2 is stored in green tanks, air in yellow tanks, and N2O in blue tanks.

Figure 3. Compressed gas color codes in the United States. Used with permission from Binda DD et al. Hospital oxygen supply: Videos in Clinical Anesthesia. Anesth Analg. 2024.3

  • A hanger yoke supports the E-cylinder and connects it to the anesthesia machine.
    • The hanger yoke includes a washer that maintains a gas-tight seal and a check valve that ensures unidirectional gas flow into the machine.2
    • Each hanger yoke also has a pin-index safety system, an important safety feature that prevents cylinder interchangeability by providing unique pin configurations for each medical gas cylinder.1
      • The pins for an O2 cylinder are found at positions 2 and 5.2
      • The pins for a N2O cylinder are found at positions 3 and 5.2

 

Figure 4. Pin index safety system for O2 and N2O, respectively. Source: Das S, et al. Indian J Anaesth. 2013;57(5):489-99. PubMed CC BY SA 3.0.6

  • The cylinder pressure regulator reduces the cylinder pressure to a lower level appropriate for the anesthesia machine.
    • The O2 cylinder pressure regulator reduces O2 pressure from up to 2200 psi to approximately 45 psi.1
    • The N2O cylinder pressure regulator reduces N2O pressure from 745 psi to approximately 45 psi.1
  • As long as the pressure in the pipeline is above 45 psi, the anesthesia machine will preferentially use the medical gas from the pipeline supply because it has a higher psi than the medical gas stored in E-cylinders.

Gas-Specific Cylinder Properties and Pressure-Volume Relationships

  • A full E-cylinder of O2 has a volume of 660 L and a pressure of 2000 psi.4
    • O2 exists in gaseous form at room temperature.
    • The volume of O2 in the cylinder is directly proportional to the pressure in the cylinder.
  • A full E-cylinder of N2O has a volume of 1,590 L and a pressure of 745 psi.2
    • N2O can exist in liquid form at room temperature because its critical temperature is 36.5°C.2 A full E-cylinder of N2O contains approximately 90 to 95% liquid N2O and 5 to 10% gaseous nitrous oxide.2
    • Once there is approximately 253 L (16%) of N2O left in the tank, the remaining N2O will exist in gaseous form.2
    • Prior to this point, the pressure will read 745 psi and will not reflect the true volume of N2O remaining in the cylinder.
    • After all the liquefied gas has been used up, the volume of N2O in the cylinder is directly proportional to its pressure.
    • The residual volume of N2O can be determined at any point by weighing the cylinder and subtracting the tare weight.2,4

 

References

  1. Riutort KT, Eisenkraft JB. The Anesthesia Workstation and Delivery Systems for Inhaled Anesthetics. In: Barash PG, Cullen BF, Stoelting RK, et al, eds. Clinical Anesthesia. 8th ed. Philadelphia, PA; Wolters Kluwer; 2017: 644 – 705.
  2. Malayaman SN, Mychaskiw G II, Berry JM, et al. Medical Gases: Storage and Supply. In: Ehrenwerth J, Eisenkraft JB, Berry JM, eds. Anesthesia Equipment: Principles and Applications. 3rd ed. St. Louis, MO; Elsevier; 2021: 3 – 24.
  3. Binda DD, Regenbogen I, Nozari A, et al. Hospital Oxygen Supply: Video in Clinical Anesthesia. Anesth Analg. 2024;138(1):221-5. PubMed
  4. The Operating Room Environment. In: Butterworth IV JF, Mackey DC, and Wasnick JD, eds. Morgan & Mikhail’s Clinical Anesthesiology. 7th ed. McGraw-Hill Education; 2022.
  5. Palaniappa NC, Weston SD. Inhaled Anesthetic Delivery Systems. In: Gropper MA, Cohen NH, Eriksson LI, et al, eds. Miller’s Anesthesia. Vol. I. 10th ed. Philadelphia, PA; Elservier; 2017: 457 – 511.e5
  6. Das S, Chattopadhyay S, Bose P. The anaesthesia gas supply system. Indian J Anaesth. 2013;57(5):489-99. PubMed

Other References

  1. Bechtel A. Wall oxygen failure, negative pressure alarm. OA Keys to the Cart. 2017. Link
  2. Young M, Diachun CA. Machine malfunction: Loss of pipeline oxygen and stuck expiratory valve. OA summary. 2026. Link
  3. Ghan T, Chatterjee D. Oxygen supply. OA summary. 2024. Link